The 49-year-old struggled to speak after the BBC fundraiser aired a truly heartbreaking story of a young girl, Vanessa.
Vanessa suffered from Neuroblastoma, a very rare type of cancer, and she spent more than 800 nights in hospital fighting the disease.
Through donations, she was able to nurture her love for singing as a professional singer visited her and they practised Les Miserables hits.
Talking about her love of music, Vanessa said: “I like to sing a lot. It’s just like another world you can go to.
“I love singing it’s fun and it relaxes me, it makes me calm when I get stressed.”
Vanessa lost her battle with cancer in September just one day after she was released from hospital to spend precious time with her friends and loved ones.
After the emotional clip aired, former Great British Bake Off host Mel had to fight back her tears as she spoke to the audience.
She said: “Now I never had the fortune to meet Vanessa but I think we can all see from that film what an exceptional girl she was and our love goes out to her family and everyone who helped her.
“And our gratitude to spread a smile because Vanessa’s smile was wonderful and it lit up the world.”
Viewers watching were also deeply affected by Vanessa’s story with the clip persuading many to donate to Children in Need.
One person wrote on Twitter: “#ChildreninNeed #Pudsey Vanessa’s story broke my heart. Much love to your family, precious girl. Sing with the angels X.”
Another said: “Vanessa’s story is heartbreaking, and it just highlights how important #ChildrenInNeed is. Such an incredible charity!”
“Wow the story of Vanessa on @BBCCiN just ripped my heart in two! What a super brave young lady! #inspiration #liveforeverymoment #lovelife #ChildrenInNeed,” added a third.
BBC Children in Need 2017: Appeal Night is now available to watch on BBC iPlayer.